Nuricell Top-grade AmineSlides

Nuricell Top-grade AmineSlides Description

Description

Nuricell Top-grade AmineSlides(NTop-AS) use a new glass that is flatter and has less thickness variation than glass used with NC-AMCS. This proprietary ultraflat glass enhances microarray performance, enabling more accurate reading of microarrays by confocal laser scanners. NTop-AS are made of special low-fluorescent glass slides with the standard size of 75 x25 x 1 mm . The overall dimensions of the slide makes it compatible with most microarray spotters and scanners. NTop-AS have very uniform coating layer and optimal density of reactive groups. Cleaning, coating and packaging of NTop-AS is performed in a Class-100 HEPA filter cleanroom . Good flatness of slide glass Low roughness of slide surface Low fluorescence glass Good spot morphology Uniform coating layer Optimal density of reactive groups High signal/noise ratio Compatible with all commercial microarray detection systems Nuricell Top-grade AmineSlides(NTop-AS) allow efficient non-covalent binding of nucleic acids, proteins and other molecules. NTop-AS contain primary amine groups (NH3+) attached covalently to the glass surface. The amines carry a positive charge at neutral pH. Nucleic acids will bind non- covalently via the negatively charged phosphate backbone. The non-covalent(electrostatic) binding of nucleic acid immobilization can be transformed into covalent coupling by UV-crosslinking or heat treatment . The combination of electrostatic binding and covalent attachment couples the DNA to the substrate is a highly stable manner .

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